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Image Search Results
Journal: Frontiers in Immunology
Article Title: Seminal Plasma and Seminal Plasma Exosomes of Aged Male Mice Affect Early Embryo Implantation via Immunomodulation
doi: 10.3389/fimmu.2021.723409
Figure Lengend Snippet: Characterization of sExos extracted from young and aged male mice. (A) Western blotting analysis on sExos using antibodies against the positive markers (TSG101, CD81, and CD9) and negative marker Albumin after BCA (Bradford) protein quantification assay. (B) Quantification of band intensity as seen in panel (A, C) Representative particle size distribution diagrams of sExos from two groups of mice as determined by nanoparticle tracking analysis. (D) Quantification of panel C (mean ± SD, n = 6). (E) TEM images of sExos isolated from young and aged male mice. Bar = 500 μm. The differences between two groups were assessed by paired-samples t-test. **p < 0.01. sExos, seminal plasma exosomes; BCA, bicinchoninic acid; TEM, transmission electron microscopy; BMDC, marrow-derived dendritic cell; SP, seminal plasma; DC, dendritic cell; ns, not significant.
Article Snippet: The following primary antibodies were used: monoclonal rabbit anti-mouse CD63 antibody (1:2,000, Abcam), monoclonal rabbit anti-mouse TSG101 antibody (1:2,000, Santa Cruz Biotechnology, Dallas, TX, USA),
Techniques: Western Blot, Marker, Isolation, Clinical Proteomics, Transmission Assay, Electron Microscopy, Derivative Assay